Threonine deaminase and acetolactate synthetase in Anabaena variabilis

Bioenergetics Studies of the Cyanobacterium Anabaena variabilis

A cell-free system exhibiting both photophosphorylation (P /2e = 1) and oxidative phosphoryla­ tion (P/O up to 0 .8 ) is described for the cyanobacterium Anabaena variabilis. NADH and N A D PH were found to be equally effective as electron donors for oxidative phosphorylation. Low concentrations of U H D B T , an inhibitor of the cytochrome blc complex of mitochondria and chloroplasts. were fou...

Potassium ion transport and electrogenesis in Anabaena variabilis.

Components of the proton electrochemical potential gradient in Anabaena variabilis.

Threonine Deaminase of Clostridium tetanomorphum

Dissociation of the threonine deaminase of Ctostridium tetanomorfihum occurs upon exposure to heat, to alkaline pH, or to denaturing agents, or when preparations are diluted or allowed to age at -20°. Dissociation in the presence of urea, detergents, or an alkaline pH may be prevented by addition of adenosine diphosphate, threonine, phosphate buffer at pH 7.0, or monovalent or divalent cations....

Threonine Deaminase of Clostridium tetanomorphum

Threonine deaminase (Lthreonine hydrolyase (deaminating), EC 4.2.1.16) has been purified approximately 700-fold from extracts of Clostridium tetanomorphum. Both threonine and serine can serve as substrates, but threonine is deaminated 5 to 10 times more rapidly than serine. Pyridoxal phosphate, a reducing agent, and alkaline pH are required for the deamination of either amino acid. A plot of th...